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(b) Whole-body imaging of GFP- and RFP-expressing tumors implanted in the brain in a single nude mouse.

For single-cell resolution, reversible skin flaps as well as chronic-transparent window models can be used over many parts of the body (skin, brain, lung, liver, etc.). Fluorescent proteins range in size from 25 to 30 k Da and form internal chromophores that do not require cofactors or substrates to fluoresce.GFP and its red, blue, yellow, and orange cousins have revolutionized bio-medical research and enabled every major disease, both cause and effect, to ‘light-up’ in the laboratory for researchers to visualize, understand, and eventually conquer them.Fluorescent proteins allow us to visualize, in real time, all important aspects of cancer in living animals, including the cancer cell cycle, mitosis, apoptosis, motility, invasion, metastasis, and angiogenesis.The image was acquired with a Hamamatsu charge-coupled device (CCD) camera.(c) An example of the best state of the art, OV100 Small Animal Imaging System (Olympus, Tokyo, Japan).

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